Description
This system is a high-content imaging system providing both spinning disk confocal and widefield imaging. It is optimized for imaging multi-well plates and has both air and water-immersion objectives. It also has temperature and CO2 for live-cell imaging.
Location
Weill Neurosciences Building
Room 451J
Contact
Caroline Mrejen, PhD
[email protected]
Hardware
- ImageXpress Confocal HT. ai High-Content Imaging System
- Andor Zyla 4.5 camera
- Dual disk unit allowing selection between 3 modes A) 60 micron pinhole disk for your basic confocal requirements B) 50 micron slit for high throughput confocal requirements and C) wide field (not-confocal) imaging mode.
- Gas Mixer
- MetaXpress Imaging and Analysis software
Objectives
Installed
- Plan Apo Lambda 4x/0.2NA (not currently installed)
- Plan Apo 10x/0.45NA
- Apo LWD 20x/0.95NA WI
- Apo LWD 40x/1.15NA WI
Not Installed
- Plan Apo Lambda 2x/0.1NA
4 positions available, WI objectives requires service engineer to exchange and calibrate.
Light Source
- 89 North LDI laser illumination
Excitation Lasers
- 405
- 445
- 475
- 520
- 555
- 640
- 730
Emission Filters
DAPI
- Excitation: 405/20 nm
- Emission: 452/45 nm
- Dichroic: FF409-Di03
CFP
- Excitation: 445/20 nm
- Emission: 483/32 nm
- Dichroic: ZT 445/520
FITC
- Excitation: 467.5/21 nm
- Emission: 520/28 nm
- Dichroic: ZT 405/470/555/640/730 or FF506-Di03
YFP
- Excitation: 520/10 nm
- Emission: 562/40 nm
- Dichroic: ZT 445/520
TRITC
- Excitation: 555 nm
- Emission: 598/25 nm
- Dichroic: ZT 405/470/555/640/730
TxRed
- Excitation: 555 nm
- Emission: 624/40 nm
- Dichroic: FF593-Di03-25
Cy5
- Excitation: 638/17 nm
- Emission: 692/40 nm
- Dichroic: ZT 405/470/555/640/730
Cy7
- Excitation: 725/30 nm
- Emission: 794/32 nm
- Dichroic: ZT 405/470/555/640/730