Description
This is a conventional widefield system for both epifluorescence and brightfield imaging and is equipped with CO2 and a temperature-controlled incubator for live-cell imaging. The fully automated system enables large image acquisition and multi-position high throughput imaging of plates. It is equipped with a Hamamatsu Flash 4.0 camera and a Leica color camera. Leica Thunder software module is also available on this system.
Location
Weill Neurosciences Building
Room 451J
Contact
Caroline Mrejen, PhD
[email protected]
Hardware
- Leica DMi8 Inverted Microscope
- Quantum High Speed linear motorized stage (Pecan)
- Hamamatsu Flash 4.0 v3
- Leica DFC9000 GTC color camera
- OkoLab Cage Incubator
- Thunder Software Package
- Computational clearing
- CAM link for Flash4
Objectives
- N PLAN 5x/0.12 PH0
- HC PL APO 10x/0.45
- HC PL APO 20x/0.80
- HC PL APO 40x/0.95 CORR
- HC PL APO 63x/1.40-0.60 OIL
Light Source
- LED 8 with Sequencer (395, 438, 475, 511, 555, 575, 635, 730 nm) with 3mm LLG
Emission Filters
405
- EX: 375-435
- DC: 455
- EM: 450-490
CYR 71010
- EX: 422-450; 495-517; 566-590; 710-750
- DC: 459; 523; 598; 763
- EM: 462-484; 527-551; 602-680; 770-850
GFP
- EX: 450-490
- DC: 495
- EM: 500-550
DFT51010
- EX: 375-407; 462-496;542-566; 622-654
- DC: 415; 500; 572; 660
- EM: 420-450; 506-532; 578-610; 666-724