CSU-W1 SoRa Spinning Disk Confocal

Description

This a super-resolution spinning disk confocal that can provide a 1.5 fold improvement in resolution over a standard spinning disk confocal. It is capable of imaging at video-rate speeds with a Photometrics Kinetex camera and is equipped with a Mightex DMD and a CO2 and temperature-controlled incubator for live-cell imaging.

Location

Weill Neurosciences Building
Room 451J

Contact

Caroline Mrejen, PhD
​[email protected]

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Hardware

  • Leica DMi8 Inverted Microscope
  • Yokagawa CSU-W1 SoRa
    • 50um disk
    • SoRa disk
    • SoRa Mag 1x, 2.8x, 4.0x
    • Zt405/488/561/640 dichroic and 100%mirror
  • Yokogawa Uniformizer
  • Photometrics Kinetix camera
  • Photometrics BSI camera
  • Mightex Spatial Illuminator
  • Microvolution Deconvolution Software
  • ASI motorized stage with Piezo top plate (500 um travel)
  • OkoLab Cage Incubator
  • LDI for mightex

Objectives

  • HC PL FL 10x/0.30
  • HC PL APO 20x/0.80
  • HC PL APO 40x/0.95 CORR
  • HCX PL APO 40x/1.10 W CORR
  • HC PL APO 100x/1.47 OIL CORR TIRF

Light Source

  • Vortran Laser Launch

Excitation Lasers

  • 401 nm
  • 487 nm
  • 561 nm
  • 639 nm

Emission Filters

Spinning Disk
  • Chroma ET 460/50
  • Chroma ET 605/70
  • Chroma ET 700/75
  • Chroma ET 525/50
  • Chroma ZET405/488/561/635 m
Widefield
  • DAPI filter cube
  • FITC filter cube
  • Rhod filter cube
  • 405nm LP Dichroic for Mightex